Nnsolid phase dna synthesis pdf files

Pdf solid phase versus solution phase synthesis of. With a solidphase chemistry approach, a large existing body of known chemical transformations becomes accessible to dnaencoded synthesis. This synthesis method is difficult to control resulting in agglomeration of the enm and is thus rarely used and will not be discussed further 32. Aristolochic acid aa is a known nephrotoxin and potential carcinogen, which can form covalent dna adducts after metabolic. In this method, building blocks are protected at all react. Solid phase synthesis of 5 triantennary nacetylgalactosamine conjugated antisense oligonucleotides using phosphoramidite chemistry. In chemistry, solid phase synthesis is a method in which molecules are covalently bound on a solid support material and synthesised stepbystep in a single reaction vessel utilising selective protecting group chemistry. Solid phase peptide synthesis, strategies and resins.

Dnabinding polyamides are synthetic oligomers of pyrroleimidazole units with high specificity and affinity for doublestranded dna. Solid phase versus solution phase synthesis of heterocyclic macrocycles article pdf available in molecules 181. Solidphase synthesis of dnaencoded libraries via an. We report chemistry suitable for the solidphase synthesis of dnaencoded libraries with an unusually high level of structural diversity. Solidphase synthesis has a number of advantages over solution synthesis. The ugi, ugiazide, and groebkeblackburnbienayme reactions are welltolerated by dna on the solid phase and show a broad scope. The strategy involves exploding an immobilized aldehyde into a plethora of different functional groups under dnacompatible conditions. A simple method for preparation and characterization of aristolochic aciddna adducts was developed. High efficiency and throughput increased simplicity and speed the reaction can be driven to completion and high yields through the use of excess reagent. Although dna exhibits limited solubility in nonaqueous solvents, its immobilization on a solid phase can be exploited to access such environments. Solid phase peptide synthesis part i applications of bocbzl strategy g bor mez research group of peptide chemistry hungarian academy of sciences powerpoint ppt presentation. Solidphase synthesis is carried out on a solid support held between filters, in columns that enable all reagents and solvents to pass through freely. Isocyanide multicomponent reactions on solidphasecoupled. Solid phase synthesis an overview sciencedirect topics.